Single-Stranded DNA is not a Worm-Like Chain

The Worm-Like Chain Theory And Bending Of Short DNA

The probability distributions for bending angles in double helical DNA obtained in all-atom molecular dynamics simulations are compared with theoretical predictions. For double helices of one helical turn and longer the computed distributions remarkably agree with the worm-like chain theory and qualitatively differ from predictions of the semi-elastic chain model. The computed data exhibit only...

WORM is not enough !

Important documents like financial reports, customer communications etc are increasingly being maintained by businesses in electronic format. These represent much of the data on which key decisions in business operations are based and hence must be maintained in a trustworthy fashion safe from destruction or clandestine modification. Secure retention of such data is also increasingly being regu...

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA

Single-stranded DNA (ssDNA) is a product of many cellular processes that involve double-stranded DNA, for example during DNA replication and repair, and is formed transiently in many others. Measurement of ssDNA formation is fundamental for understanding many such processes. The availability of a fluorescent biosensor for the determination of single-stranded DNA provides an important route to a...

Building a Modular Single-Stranded DNA Production Device Using Multi-Copy Single-Stranded DNA

Intracellular viral processing, not single-stranded DNA accumulation, is crucial for recombinant adeno-associated virus transduction.

Adeno-associated virus (AAV) is a unique gene transfer vector which takes approximately 4 to 6 weeks to reach its expression plateau. The mechanism for this slow-rise expression profile was proposed to be inefficient second-strand DNA synthesis from the input single-stranded (ss) DNA viral genome. In order to clarify the status of ss AAV genomes, we generated AAV vectors labeled with bromodeoxy...